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It has been suggested this claim evolved out of the real observation that certain species of fish in the Amazon will gather at the surface near the point where a urine stream enters, having been attracted by the noise and agitation of the water.
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Martius also speculated that the fish were attracted by the "odor" of urine.
Another report from French naturalist Francis de Castelnau in 1855 relates an allegation by local Araguay fisherman, saying that it is dangerous to urinate in the river as the fish "springs out of the water and penetrates into the urethra by ascending the length of the liquid column." While Castelnau himself dismissed this claim as "absolutely preposterous," and the fluid mechanics of such a maneuver defy the laws of physics, it remains one of the more stubborn myths about the candiru.
Candirus (Vandellia) inhabit the Amazon and Orinoco basins of lowland Amazonia, where they constitute part of the Neotropical fish fauna.
Candirus are hematophagous and parasitize the gills of larger Amazonian fishes, especially catfish of the family Pimelodidae (Siluriformes).
These smaller species are known for an alleged tendency to invade and parasitise the human urethra; however, despite ethnological reports dating back to the late 19th century, Candirus are small fish.
Adults can grow to around 40 centimetres (16 in) with a rather small head and a belly that can appear distended, especially after a large blood meal.
He relates that the fish was extracted after external and internal application of the juice from a Xagua plant (believed to be a name for Genipa americana).